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Excalibur, the very symbol of King Arthur's power has been stolen. Unable to immediately pursue the thief, Arthur and Merlin travel north to mediate a dispute over the dismantling of Hadrian's wall. Queen Gwenhwyvar and Sorceress Morgan Le Fay take it upon themselves to recover the stolen sword. Little do they know a magical beast guards the sword and will devour any who try to retrieve it.
Guinevere the Queen AD494 (AD 491 - AD 517) Aenghus Chisholme
Otherwise - IPTGBL21-AI Invitrogen BL21-AI was constructed by inserting a chromosomal copy of the T7 RNA polymerase gene under the tight control of the arabinose-inducible araBAD promoterChloramphenicol L-rhamnose (different concentratins), see protocol Tuning ETetracyclin 12.5ug/ml The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction
The cytoplasmic DsbC is also a chaperone that can assist n the folding of proteins that do not require disulfide bondsBL21(DE3) Novagen DE3 lysogen contains T7 polymerase upon IPTG inductionThe pLysS plasmid produces T7 lysozyme to reduce basal level expression of the gene of interestOrigami B pLysS Novagen Kanamycin 15ug/ml Tetracyclin 12.5ug/ml Chloramphenicol 34ug/ml For protein expression: Gentamycin 20ug/mL For transformation: no need for antibiotic other than the one of the transformed plasmid.
Chloramphenicol 34ug/ml PDF DsbC promotes the correction of mis-oxidized proteins into their correct formChloramphenicol 34ug/ml Rosetta-gami-pLysS Novagen Rosetta-gami host strains are Origami derivatives that combine the enhanced disulfide bond formation resulting from trxB/gor mutations with enhanced expression of eukaryotic proteins that contain codons rarely used in EBacterial Strain Company Features Growth Requirements Documentation [pLysS contains the p15A origin
C41(DE3) Lucigen Effective in expressing toxic and membrane proteins from all classes of organisms,including viruses,eubacteria,archaea,yeasts,plants,insects,and mammalsLower level expression may enhance the solubility and activity of difficult target proteinsThe pLysS presence allows tight control of expressionUse this strain for both soluble and insoluble protein productionProtocol
For difficult soluble proteins, tuning the expression level may also result in more soluble, properly folded proteinBL21 Star-pLysS Invitrogen This strain is mutated in Rnase E, thus Rnase degradation is reduced, and protein expression may be increasedDetailed description & protocols (PDF) None Suitable for T7 promoter driven protein expression 171bf2437f